Pro5 MHC Class I Pentamers | ProImmune

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Pro5® MHC Class I Pentamers

The most consistent technology for detecting antigen-specific T cells

If you are trying to detect a rare population of antigen specific T cells you will understand the importance of having a research tool that is sensitive, accurate and gives reproducible results.

Pro5® MHC Class I Pentamers provide the answer. They bind directly to T cell receptors of a particular specificity, determined by the Major Histocompatibility Complex (MHC) allele and peptide combination. Pro5 Pentamers can be used to detect and separate antigen-specific CD8+ T cell populations as rare as 0.02% of lymphocytes. They are also suitable for detailed epitope characterization and further immune monitoring.

Unlike tetramers from academic and non-profit sources, which may be of variable quality and stability, Pro5 Pentamers benefit from a superior design and are made to a professional standard including many stringent quality control steps. Using the Pentamer technology enables you to achieve accurate and reproducible results.

There are many reasons to choose ProImmune's products for your antigen-specific CD8+ T cell research

  • Pro5® MHC Class I Pentamers deliver consistent and reproducible results

  • Applications data and support: customers rely on our detailed protocols and technical experience to support and enhance their research

  • Fastest growing list of publications: researchers are citing Pro5® Pentamers in publications more often than MHC tetramers or MHC multimers from any other commercial source

 

Features of Pro5® MHC Class I Pentamers

Consistency and reproducibility of results

High avidity of binding to T cell receptor

Flexibility in choice of fluorescent label

Brighter overall fluorescence

Widest range of available complexes

Long term storage

Pro5® Pentamers comprise five MHC-peptide complexes assembled through a coiled-coil domain. Due to their planar configuration, all five MHC-peptide complexes in the Pentamer are available for binding to complementary T cell receptors (TCRs). Each Pro5® Pentamer also comprises up to five fluorescent or biotin tags for bright and efficient labeling. In contrast, the MHC tetramer technology relies on MHC-peptide complexes held in a tetrahedral complex where due to spatial organization no more than three MHC-peptide complexes are available for TCR-binding.

A broad range of MHC Class I complexes specific for a wide range of infectious diseases such as Influenza, CMV, hepatitis B and C, HIV, EBV, as well as cancer-related epitopes are available as Pro5® Pentamers. Simian alleles, Mamu-A*01 and Mamu-A*02 are available for SIV research. Click here to view our full range of almost 300 Pentamers. Additionally, ProImmune has many years experience making Custom MHC Pentamers, specific to customers' requirements and with a high rate of success.

ProImmune has extensive experience in testing Pro5® Pentamer products in-house in a variety of applications. This enables us to support our customers fully with their relevant application requirements, helping them to make best use of our technology.

Advantages of Pro5® MHC Class I Pentamers

 

Pro5® Pentamers

Tetramers

Avidity &
configuration

5 MHC-peptide complexes per Pentamer in the same orientation result in enhanced avidity interaction with T cell receptor (TCR)

MHC-peptide complex held in a tetrahedral configuration typically allowing only 3 MHC-peptide complexes to bind to TCRs

Brightness

Unique labeling technology allows up to 5 fluorescent labels to attach to each Pentamer, yielding brighter signal

By nature, tetramers will have one fluorescent label located at the center of the complex

Labeling flexibility

Biotin-labeled Pentamers and unlabeled Pentamers, which can be labeled with the fluorochrome of choice, maximize the range of possible applications

Tetramers, made by multimerizing biotinylated MHC monomers with fluorescent labeled streptavidin, must be supplied pre-labeled

Stability

Unlabeled Pentamers can be stored long-term at -80°C and then used when required

Tetramers incorporating a fluorochrome must be stored at 4°C, reducing their shelf life

Figure: MHC multimer staining vs. CD8 FITC of peripheral blood (PBMC) samples. 1 x 105 PBMCs with specificity for each antigen shown were stained with either Pro5® Pentamer or MHC tetramer. The figure shows live cells and demonstrates the sensitivity and increase in mean fluorescence intensity when cells are stained with Pentamer vs. tetramer.

Application Support for Pro5® MHC Class I Pentamers

Flow Cytometry

Co-staining with Intracellular Cytokines

Magnetic Bead Cell Separation

Tissue Staining

 

Pro5® MHC Class I Pentamer Sample Product Sheets

Pro5® MHC Class I Pentamer - APC Labeled

Pro5® MHC Class I Pentamer - Biotin Labeled

Pro5® MHC Class I Pentamer - A0201 Negative Control

Pro5® MHC Class I Pentamer - R-PE Labeled

Pro5® MHC Class I Pentamer - Unlabeled

Pentamers in more detail