ProImmune REVEAL® Class II Rapid Epitope Discovery System

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Mouse Class II MHC-Peptide Binding Assays

HLA-Peptide Binding Assays as part of Immunogenicity Risk Management

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ProImmune REVEAL® MHC-Peptide

Competition Binding Assays


How Strong is Your Competitor?

There are situations when you need to evaluate MHC-binding of peptides not just on its own but how a peptide or peptide-mimetic competes with a known control peptide for binding. 

As an extension to our regular ProImmune REVEAL® MHC-peptide binding and rate assays we are now also offering MHC-peptide competition binding assays.


These new assays are currently available for the following HLA-DR supertypes:

HLA-DRB1*01:01, *03:01, *04:01, *04:05, *07:01, *09:01, *11:01, *15:01.

Like our other specialist assays our competition assays turn around rapidly in only a few weeks.



ProImmune REVEAL® Competition Assay Principle



Figure 1. Competition assay principle: A labelled control peptide is bound to the MHC-complex, giving a high baseline signal in an immunoassay. As the peptide is competed away with unlabelled competitor peptide the signal reduces at increasing concentration of competitor peptide. The control peptide is usually a well known HLA-Class II restricted immunodominant T cell epitope which is further known to be restricted to the HLA-DR supertype of interest.



Figure 2. Example data for the assay. Half-life is determined using 12 competitor concentrations and single time constant off rate curve.



Additional Features:

The assay can be adapted to accommodate a range of requirements:

Control peptides can be chosen depending on the assay objective

Test peptide or compounds provided by customer or made by ProImmune

The concentration range can be varied to suit the specific project objective

Mixtures of peptides as well as mono-disperse peptides can be tested


Our Immunology Specialist Sales Team will provide a quotation tailored to your specific project requirements.

Key publication for ProImmune REVEAL® Competition Assay:


Immunotherapy with apitopes blocks the immune response to TSH receptor in HLA-DR transgenic mice

Liselotte Jansson, Kathleen Vrolix, Andrea Jahraus, Keith Martin, David Wraith

Endocrinology 159: 3446–3457, 2018