Managing Immunogenicity

Cell-mediated Immunity Assays

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The Special Assay Service

Bespoke immunology assays based on ProImmune’s unique platform technologies ProImmune offers unique assays with a broad range of applications in research immunology, including:      • Immune modulation assessment      • Managing drug immunogenicity risk      • Characterizing responses to new antigens      • All aspects of immune monitoring      • Comparison of patients to healthy donors

Researchers often approach us to ask for bespoke assays. Usually our standard assays can be readily adapted to suit your requirements. Our customer service team are Ph.D-educated immunologists, able to understand your requirements in detail, and work with you to find the most effective solution to your needs.

We call this our 'Special Assay Service'.

Our standard assays include:

• Measuring antigen presentation by dendritic cells by identifying peptides on the cell surface

• Dendritic cell culture and differentiation

• T cell and Dendritic cell : T cell proliferation assays

• An extensive range of MHC class I and class II peptide binding assays

• T and B cell ELISpot assays

• Flow cytometric assays including intracellular staining

• Protein and Peptide microarrays

• Tissue Typing

• Cell processing and storage for cellular assays

We can use any of these experimental platforms as a basis on which to build a custom assay for your needs. For example, at the same time as measuring T cell proliferation, we can measure cytokine release and T cell differentiation, bringing several of our areas of expertise together. Assays like these could take months for you to develop and qualify in-house, but for us, adapting a standard protocol is something we’re used to doing, usually in a matter of days.

Our custom assays
How have we helped our clients so far?

The majority of the custom work we perform is confidential, but in the last year we have carried out a collection of studies including:

Sourcing 20 donors with a known vaccination status to carry out ELISpot testing for T cell responses to a collection of proteins (7 weeks from order to delivery).

A modified version of our MHC-binding assay to derive detailed MHC-binding information for a biologic (8 weeks from order to delivery).

A T cell proliferation assay on donors of a specific HLA type, following both CD4+ and CD8 + T cell responses in the same assay (5 weeks from order to delivery).

Contact us to discuss your ideas, and combine them with ours.