typeHLA - HLA Typing Simplfied
Over 99% of our genes are the same
HLA type is a crucial part of the difference
HLA Tissue Typing Service
Why Should You Outsource HLA Tissue Typing to ProImmune?
HLA Tissue Typing Customer Reviews
Dr. Jennifer Kirchherr at the Duke Human Vaccine Institute in North Carolina, USA affirms the importance of correlating the immune response observed with HLA tissue type for their researchers carrying out clinical studies. In addition she comments, “We looked at a range of companies offering tissue typing, and also at using an on-campus typing facility, and we found that ProImmune were able to offer us the best pricing and turnaround time.” Dr. Kirchherr and her colleagues send regular batches of samples to ProImmune for typing. “They have been wonderfully easy to work with, if we ask anything they get right back to us, and overall it has been a really good experience” she added.
Highlighting the relevance of tissue typing, and the global availability of typeHLA to researchers all over the world, Dr. Navapon Techakriengkrai at Chulalongkorn University, Thailand has also had success with the service. “I outsource my tissue typing to ProImmune because they provide me with the fastest service at the best price. Since the main theme of my research is on T cell immunology, HLA-type is inevitably needed. The customer service from ProImmune is of the best quality. Their tissue typing service allowed me to unambiguously resolve the HLA type of many of my samples.”
Tier-1, Class I and Class II
Typing by PCR-sequence specific oligonucleotides (PCR-SSOP) to resolve major allele groups to 4 digits, with some degeneracy e.g. HLA-A*23:01/03/05/06).
PCR-SSOP: The genomic DNA is amplified using PCR, then incubated with a panel of different oligonucleotide probes, which have distinctive reactivities with different HLA-types. The Luminex xMAP® technology is used, where oligonucleotide probes are individually attached to up to 100 distinctly fluorescent microspheres. This allows the measurement of 100 different reactions in a single tube.
Tier 2, Class I and Class II
Can be carried out if needed following Tier 1 typing to achieve higher resolution. Typing by PCR-sequence specific primers (PCR-SSP) or PCR-sequencing, will usually resolve to a specific 4-digit allele, with only occasional degeneracy.
PCR-SSP: the PCR reaction is used to define whether the targeted HLA allele is present or absent by using reagents in the PCR reaction specific for individual HLA alleles.
PCR-sequencing: the DNA sequence of the HLA allele can be directly analyzed by performing nucleotide sequence analysis of the amplified DNA.
Sample Types and Order Handling
We accept genomic DNA, saliva samples, fresh whole blood or frozen cells, and will provide you with details of how to ship each of these to us. There is an order handling charge for samples sent as whole blood or frozen cells due to the additional processing needed for these sample types.
We offer a worldwide service, available for one sample or hundreds - our high throughput service enables us to process tens to hundreds of samples at a time. You can confidently send your samples from any location worldwide using our experience in shipping globally. We can accept genomic DNA, saliva, whole blood or cryopreserved PBMCs.