HLA Tissue Typing Service | ProImmune

HLA Tissue Typing Service from ProImmune

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typeHLA - HLA Typing Simplified

Over 99% of our genes are the same

HLA type is a crucial part of the difference

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HLA Tissue Typing Service

  • Straightforward, dependable core facility HLA tissue typing service

  • Using state of the art genotyping technologies as used in HLA typing for organ transplantation

  • We work with genomic DNA, Saliva, Whole Blood, or Cryopreserved cells

  • Detailed results typically sent in 3 weeks

 

typeHLA Tissue Typing Service Overview

 

Typing technology options

New Next Generation Sequencing (NGS)

PCR-SSOP using Luminex®

(previously called Tier 1)

HLA Class I loci available

A, B and C

(whole Class I panel reported)

A, B, C

(can be ordered individually)

HLA Class II loci available

DRB1, DPB1 and DQB1

(whole Class II panel reported)

DRB1, DRB3,4,5, DPA1*, DPB1, DQA1*, DQB1

(can be ordered individually)

Resolution of typing data

 

Fully resolved 4 digit (allelic level) typing with no degeneracy for all samples

 

 

4 digit (allelic level) typing but with some degeneracy

 

Features / Restrictions

Only available for ordering whole Class I panel (3 loci) or whole Cass II panel (3 loci) or whole Class I and Class II panel (6 loci) Can be ordered for each locus individually

Turnaround time (approximate)

3 weeks

Sample formats accepted

gDNA, Cryopreserved PBMCs/other Cells, Blood, Saliva

Report format

Electronic format (PDF, XLS) via secure webserver

 

* DQA1 and DPA1 typing is provided free of charge with DQB1 and DPB1 typing, respectively, where possible.

 

typeHLA Tissue Typing Service in Detail

Option 1: NGS (Next Generation Sequencing), Class I and Class II

Allelic-level typing of full-length HLA genes ensures 4-digit typing is obtained, with no requirement for additional testing to resolve ambiguities. Where prior art PCR-SSOP typing may return a type of: HLA-A*23:01/03/05/06, NGS would determine the exact allele present - e.g. HLA-A*23:01. 

In NGS Typing, full length HLA genes are sequenced, and sequence data is phased, meaning that the complete sequences of both copies of the gene are known. Next Generation Sequencing therefore removes cis-trans ambiguities - where an HLA type cannot be confirmed due to a lack of information on which copy of the gene carries particular polymorphisms.

NGS HLA typing allows you to determine HLA types precisely, quickly, and in a single analysis, saving time and money, and removing doubt from HLA analysis.

 

Option 2: PCR-SSOP Typing (previously Tier 1), Class I and Class II

Typing by PCR-sequence specific oligonucleotide probes (PCR-SSOP) to resolve major allele groups to 4 digits, with some degeneracy in allele resolution, e.g. HLA-A*23:01/03/05/06.

In PCR-SSOP the genomic DNA is amplified using PCR, then incubated with a panel of different oligonucleotide probes, which have distinctive reactivities with different HLA-types. Luminex xMAP® technology is used, where oligonucleotide probes are individually attached to up to 100 distinctly fluorescent microspheres. This allows the measurement of 100 different reactions in a single tube.

 

Sample Types and Order Handling

We accept genomic DNA, saliva samples, fresh whole blood or frozen cells, and will provide you with details of how to ship each of these to us. There is an order handling charge for samples sent as saliva, whole blood or frozen cells due to the additional processing needed for these sample types.

We offer a worldwide service, available for one sample or hundreds - our high throughput service enables us to process tens to hundreds of samples at a time. You can confidently send your samples from any location worldwide using our experience in shipping globally.

 

Why Should You Outsource HLA Tissue Typing to ProImmune?

  • Professional, reliable HLA Tissue Typing service;

  • Avoids issues that can arise from informal relationships with a local typing lab;

  • Uses state of the art genotyping technologies as used in HLA typing for organ transplantation

  • In advance of starting functional cellular assays such as ELISpot, or carrying out flow cytometry testing with HLA multimers, tissue typing is usually required for interpreting results successfully;

  • If you are carrying out a clinical trial, establishing the tissue type of your donor samples is an important step. Many diseases and the immunological response to them are associated with specific HLA types. Correlating your trial results with the sample tissue type may give you critical additional information that could make a difference to the interpretation of the results;

  • FDA Guidance on Immunogenicity Assement for Therapeutic Protein Products (August 2014) recommends as follows:  "Evalulation of genetic factors that may modulate the immune response to a  therapeutic protein product is recommended. For example, the subset of patients that generate neutralizing antibodies to IFN-beta products are more likely to possess distinct HLA haplotypes (Hoffmann, et al. (2008) Am J Hum Genet, 83(2):219-27). Thus knowledge of the heightened susceptibility of patients with such HLA haplotypes may allow for measures to prevent such responses or to pursue other treatment options."

  • If your time and resources are limited, and deadlines are imminent, our typeHLA Service provides just the help you need.

 

HLA Tissue Typing Customer Reviews

Dr. Jennifer Kirchherr at the Duke Human Vaccine Institute in North Carolina, USA affirms the importance of correlating the immune response observed with HLA tissue type for their researchers carrying out clinical studies. In addition she comments, “We looked at a range of companies offering tissue typing, and also at using an on-campus typing facility, and we found that ProImmune were able to offer us the best pricing and turnaround time.” Dr. Kirchherr and her colleagues send regular batches of samples to ProImmune for typing. “They have been wonderfully easy to work with, if we ask anything they get right back to us, and overall it has been a really good experience” she added.

Highlighting the relevance of tissue typing, and the global availability of typeHLA to researchers all over the world, Dr. Navapon Techakriengkrai at Chulalongkorn University, Thailand has also had success with the service. “I outsource my tissue typing to ProImmune because they provide me with the fastest service at the best price. Since the main theme of my research is on T cell immunology, HLA-type is inevitably needed. The customer service from ProImmune is of the best quality. Their tissue typing service allowed me to unambiguously resolve the HLA type of many of my samples.”

 

Image of sample typing report

Discuss your requirements with our customer service team of highly trained immunologists. They will work out the best service for you, and manage the whole process from sample handling to delivery of the final report.

Typically, the results report is available 15 working days following receipt of the samples and is downloaded by the client through our secure server.

ProImmune's Cellular Analysis Services
ELISpot assays, Intracellular Cytokine Staining, CFSE T Cell Proliferation assays and Flow Cytometry staining with MHC Class I Pentamers.