HLA Tissue Typing Service | ProImmune

HLA Tissue Typing Service from ProImmune

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ProImmune's Cellular Analysis Services

Sample types for Tissue Typing

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typeHLA - HLA Typing Simplified

Over 99% of our genes are the same

HLA type is a crucial part of the difference

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HLA Tissue Typing Service

  • Straightforward, dependable core facility HLA tissue typing service

  • We work with genomic DNA, Saliva, Whole Blood, or Cyropreserved cells

  • Carried out using the most up-to-date genotyping technologies

  • Tier 1 Typing using PCR-SSOP, resolves major allele groups to 4 digits, with some degeneracy

  • Tier 2 Typing using PCR-SSP or PCR-sequencing for higher resolution

  • Order any or all of the following loci: Class I (A, B, C) and Class II (DRB1, DRB3/4/5, DPB1, DQA1&DQB1)

  • Detailed results typically sent in 15 working days

 

Why Should You Outsource HLA Tissue Typing to ProImmune?

  • Professional, reliable HLA Tissue Typing service;

  • Avoids issues that can arise from informal relationships with a local typing lab;

  • In advance of starting functional cellular assays such as ELISpot, or carrying out flow cytometry testing with HLA multimers, tissue typing is usually required for interpreting results successfully;

  • If you are carrying out a clinical trial, establishing the tissue type of your donor samples is an important step. Many diseases and the immunological response to them are associated with specific HLA types. Correlating your trial results with the sample tissue type may give you critical additional information that could make a difference to the interpretation of the results;

  • FDA Draft Guidance on Immunogenicity Assement for Therapeutic Protein Products (Feb 2013) recommends as follows:  "Evalulation of genetic factors that may modulate the immune response to a  therapeutic protein product is recommended. For example, the subset of patients that generate neutralizing antibodies to IFN-beta products are more likely to possess distinct HLA haplotypes (Hoffmann, et al. (2008) Am J Hum Genet, 83(2):219-27). Thus knowledge of the heightened susceptibility of patients with such HLA haplotypes may allow for measures to prevent such responses or to pursue other treatment options."

  • If your time and resources are limited, and deadlines are imminent, our typeHLA Service provides just the help you need.

 

HLA Tissue Typing Customer Reviews

Dr. Jennifer Kirchherr at the Duke Human Vaccine Institute in North Carolina, USA affirms the importance of correlating the immune response observed with HLA tissue type for their researchers carrying out clinical studies. In addition she comments, “We looked at a range of companies offering tissue typing, and also at using an on-campus typing facility, and we found that ProImmune were able to offer us the best pricing and turnaround time.” Dr. Kirchherr and her colleagues send regular batches of samples to ProImmune for typing. “They have been wonderfully easy to work with, if we ask anything they get right back to us, and overall it has been a really good experience” she added.

Highlighting the relevance of tissue typing, and the global availability of typeHLA to researchers all over the world, Dr. Navapon Techakriengkrai at Chulalongkorn University, Thailand has also had success with the service. “I outsource my tissue typing to ProImmune because they provide me with the fastest service at the best price. Since the main theme of my research is on T cell immunology, HLA-type is inevitably needed. The customer service from ProImmune is of the best quality. Their tissue typing service allowed me to unambiguously resolve the HLA type of many of my samples.”

 

ProImmune's typeHLA Tissue Typing Service in Detail

Tier-1, Class I and Class II

Typing by PCR-sequence specific oligonucleotides (PCR-SSOP) to resolve major allele groups to 4 digits, with some degeneracy e.g. HLA-A*23:01/03/05/06).

PCR-SSOP: The genomic DNA is amplified using PCR, then incubated with a panel of different oligonucleotide probes, which have distinctive reactivities with different HLA-types. The Luminex xMAP® technology is used, where oligonucleotide probes are individually attached to up to 100 distinctly fluorescent microspheres. This allows the measurement of 100 different reactions in a single tube.

Tier 2, Class I and Class II

Can be carried out if needed following Tier 1 typing to achieve higher resolution. Typing by PCR-sequence specific primers (PCR-SSP) or PCR-sequencing, will usually resolve to a specific 4-digit allele, with only occasional degeneracy.

PCR-SSP: the PCR reaction is used to define whether the targeted HLA allele is present or absent by using reagents in the PCR reaction specific for individual HLA alleles.

PCR-sequencing: the DNA sequence of the HLA allele can be directly analyzed by performing nucleotide sequence analysis of the amplified DNA.

Sample Types and Order Handling

We accept genomic DNA, saliva samples, fresh whole blood or frozen cells, and will provide you with details of how to ship each of these to us. There is an order handling charge for samples sent as whole blood or frozen cells due to the additional processing needed for these sample types.

We offer a worldwide service, available for one sample or hundreds - our high throughput service enables us to process tens to hundreds of samples at a time. You can confidently send your samples from any location worldwide using our experience in shipping globally. We can accept genomic DNA, saliva, whole blood or cryopreserved PBMCs.

Image of sample typing report

Discuss your requirements with our customer service team of highly trained immunologists. They will work out the best service for you, and manage the whole process from sample handling to delivery of the final report.

Typically, the results report is available 15 working days following receipt of the samples and is downloaded by the client through our secure server.

ProImmune's Cellular Analysis Services
ELISpot assays, Intracellular Cytokine Staining, CFSE T Cell Proliferation assays and Flow Cytometry staining with MHC Class I Pentamers.