Transgenic mouse models have become an important tool with which to evaluate vaccinations with putative human antigens. HLA-A2 transgenic mice express modified class I MHC molecules combining the alpha-1 and alpha-2 domains of HLA-A*02:01 with the alpha-3 domain of H-2Kb.

In order to study the cytotoxic T cell immune response in such mice it has been suggested that chimeric A2Kb multimers can be used in order to detect accurately the relevant immune responses.