Figure 1. HIV-1 gag p17/p24 with three epitopes from the HIV ProMix™ peptide pool mapped to its surface.
ProMix™ peptide pools from thinkpeptides consist of a select number of peptides representing the key immunodominant epitopes from a specific protein or pathogen, providing a comprehensive picture of the T cell response. Peptide selection is based on well-established and published epitopes with a wide range of HLA restrictions covering the most relevant HLA types in the population.
Unlike peptide pools of protein spanning overlapping peptide libraries, ProMix™ pools allow for a much greater control and purity of its constituent peptide components and avoid overloading cell cultures with large numbers of irrelevant non-responding peptides that can contribute excessive contaminants from peptide synthesis processes.
Anikeeva N et al.(2016). “Evaluating frequency and quality of pathogen-specific T cells”
Nature Communications DOI: 10.1038/ncomms13264
Brief synopsis: Using synthetic peptides and peptide pools Anikeeva et al. have developed a Ca2+ signalling activation assay that offers new insights into the dynamics of antigen-specific T cell activation.
Figure 1. Kinetics of calcium response of CMV-specific CD8+ T cells.CMV-specific CD8+ T cells from a healthy donor (a) and a patient after haploidentical stem cell transplantation (b) revealed different kinetics of Ca2+ signalling on antigen stimulation. CD8+ T cells were purified from PBMC by negative magnetic sorting and purified CD8 T cells were labelled with Fluo-4 and immobilized on a glass surface as described in ‘Methods’ section. The T cells from healthy donor were stimulated by CMV-derived peptide NLVPMATV at 10−4 M while the T cells from the patient were triggered with ProMix CMV peptide pool at 9 × 10−5 M. The images of responding cells are taken at 720 s after the stimulation. The data shown are representative of 5 (a) and 2 (b) independent experiments and are based on the analysis of 4,600 and 4,200 T cells, correspondingly. Scale bars, 50 μm.
With ProMix™ peptide pools, the pooling procedure is done for you, with the peptides guaranteed >95% pure. Each ProMix™ pool contains 25μg of each peptide and is available for dispatch within 1-2 days.
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