Download protocol PDFs from the links below:
Labeled Pentamer staining protocol
Unlabeled Pentamer staining protocol (applicable for standard unlabeled Pentamers and ProVE® Pentamers)
Biotin-labeled Pentamer staining protocol
Pentamer staining for whole blood
Staining a single cell sample with multiple labeled Pentamers
Staining a single cell sample with multiple unlabeled Pentamers
Pentamer immunohistochemistry protocol
Human Pentamer and intracellular cytokines
Murine Pentamer and intracellular cytokines
CD1d Tetramer staining protocol
Column-based bead isolation for fluorescent Pentamers
Column-based bead isolation for biotin-labeled Pentamers
Tube-based bead isolation for biotin-labeled Pentamers
CD137 isolation and staining protocol
Protocol for red blood cell depletion from whole blood
Protocol for density gradient separation of human PBMCs from blood
Protocol for the isolation of splenocytes from murine spleens
Cryopreservation of cell samples
Protocol for the isolation and expansion of antigen-specific cells
Storage and solubilization of peptides
PEPscreen®: Custom Peptide Libraries Storage and Handling Guidelines
Guidelines for dissolving PEPscreen® Peptide Sets